Transgenic Vector and Line Development Specialist - 137596

Under supervision, perform experimental procedures at the full operational level to develop a stable transgenic line in a marine invertebrate model. Perform advanced molecular cloning, functional genomics, and precision micromanipulation techniques to support ongoing research objectives. Manage the entire experimental workflow from initial construct design and optimization, through embryo microinjection, to establishing and maintaining stable transgenic animal lines. Refine and adapt standard procedures, demonstrating ingenuity, resourcefulness, and adaptability in response to specialized and evolving research needs.

• Bachelor's Degree (B.S.) in Molecular Biology, Genetics, Biochemistry, or a closely related field, or an equivalent combination of education and experience.

• Theoretical knowledge and practical experience with modern gene editing and transgenesis techniques, including CRISPR/Cas9 systems and transposase technology.

• Demonstrated, high-level work experience making or establishing stable transgenic lines of any animal.

• Demonstrated advanced skill and practical knowledge in complex molecular cloning, plasmid design, and vector engineering for fluorescent reporters/biosensors.

• Demonstrated practical experience using fluorescence microscopy to screen and image fluorescent reporters (e.g., GFP, RFP) in cells or organisms.

• Demonstrated practical experience and high level of manipulative skill in microinjecting embryos or oocytes of any animal species.

• Demonstrated ability to apply basic bioinformatics tools (e.g., BLAST, sequence alignment) to gene annotation and design precise molecular constructs in non-model systems.

• Proven ability to carry out complex procedures with limited supervision and use ingenuity and resourcefulness to adapt and optimize standard techniques for non-model organisms.

• Demonstrated ability to interact using tact and diplomacy with diverse personalities including academic, staff, student, and volunteer employees and institutions/organizations. Ability to effectively communicate and interact with a variety of people from the public to governmental agencies.

• Job offer is contingent upon satisfactory clearance based on background check results.

• The live-animal nature of this research may require work outside regular business hours, including evenings and weekends. Flexible scheduling is offered to accommodate these requirements.

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