Rsch Pro 2-Biochemistry

Summary of Position
Perform and troubleshoot experiments and analyze data in a molecular biology and protein biochemistry / protein engineering laboratory. Maintain detailed scientific records. Collaborate with the Research Team Scientists to meet Laboratory Objectives and Key Results (OKRs).

Perform all activities with minimal supervision (weekly meetings with supervisor to review progress but day to day activities performed independently) in accordance with standard operating procedures. Maintain all associated laboratory equipment and facilities for the continued performance of these duties.

30% Molecular Biology and Cell Culture
Execute and troubleshoot complex molecular biology work flows including Gibson assembly-based sub-cloning and multi-fragment assembly, PCR, Sanger sequencing of plasmids, designing cloning strategies, phage-display and yeast-display-based molecular engineering.

Perform all activities with minimal supervision (weekly meetings with supervisor to review progress but day to day activities performed independently) in accordance with standard operating procedures. Maintain all associated laboratory equipment and facilities for the continued performance of these duties.

30% Protein biochemical and biophysical assays
Execute and troubleshoot complex protein purification work flows affinity purification of recombinant proteins, size-exclusion chromatography of recombinant proteins, and ion-exchange chromatography of recombinant proteins. Validate protein identity by mass-spectrometry and perform functional assays on purified proteins including (for example) enzyme-activity assays, protein-ligand binding assays, and stopped-flow-based measurements of biochemical reaction kinetics.

Perform all activities with minimal supervision (weekly meetings with supervisor to review progress but day to day activities performed independently) in accordance with standard operating procedures. Maintain all associated laboratory equipment and facilities for the continued performance of these duties.

30% Recombinant Protein Expression and Purification
Perform cell culture, developing, maintaining, and validating cell lines and strains including maintaining and culturing HEK cells in a dedicated BSL2 cell culture facility.

Perform all activities with minimal supervision (weekly meetings with supervisor to review progress but day to day activities performed independently) in accordance with standard operating procedures. Maintain all associated laboratory equipment and facilities for the continued performance of these duties.

10% General Laboratory Maintenance
Maintain laboratory notebooks, laboratory records and databases, and lead or assist in general laboratory maintenance.

Minimum Qualifications:
BA/BS and 1 year of related experience or a combination of related education and work experience to equal five years in any of the following areas: Microbiology, Chemistry, Biochemistry, Biology, Pharmacology, or related field.

PREFERRED QUALIFICATIONS:
BA/BS and 1 year of related experience or a combination of related education and work experience to equal four years in Molecular Biology, Mammalian Cell culture, or Protein expression and purification experience.

BMBB:

The department of Biochemistry, Molecular Biology and Biophysics is committed to understanding the molecular mechanisms of metabolic diseases and cancer; developing novel strategies in biocatalysis and biotechnology; and advancing knowledge through structural biology and molecular biophysics. We organize ourselves into research divisions with emphases on Chemical and Structural Biology, Metabolic and Systems Biology, Molecular Biology and Synthetic Biology and Biotechnology. We are housed in new buildings with state-of-the-art research and support facilities.

Pay Range: 20.11 - 21.64/hourly; depending on education/qualifications/experience

Time Appointment: 100% Appointment

Position Type: Civil-Service & Non-Faculty Labor Represented Staff

Please visit the Office of Human Resources website for more information regarding benefit eligibility.

The University offers a comprehensive benefits package that includes:

• Competitive wages, paid holidays, and generous time off
• Continuous learning opportunities through professional training and degree-seeking programs supported by the Regents Tuition Benefit Program
• Low-cost medical, dental, and pharmacy plans
• Healthcare and dependent care flexible spending accounts
• University HSA contributions
• Disability and employer-paid life insurance
• Employee wellbeing program
• Excellent retirement plans with employer contribution
• Public Service Loan Forgiveness (PSLF) opportunity
• Financial counseling services
• Employee Assistance Program with eight sessions of counseling at no cost
• Employee Transit Pass with free or reduced rates in the Twin Cities metro area

Applications must be submitted online. To be considered for this position, please click the Apply button and follow the instructions. You will be given the opportunity to complete an online application for the position and attach a cover letter and resume.

Additional documents may be attached after application by accessing your "My Job Applications" page and uploading documents in the "My Cover Letters and Attachments" section.

To request an accommodation during the application process, please e-mail employ@umn.edu or call (612) 624-8647.

The University recognizes and values the importance of diversity and inclusion in enriching the employment experience of its employees and in supporting the academic mission. The University is committed to attracting and retaining employees with varying identities and backgrounds.

The University of Minnesota provides equal access to and opportunity in its programs, facilities, and employment without regard to race, color, creed, religion, national origin, gender, age, marital status, disability, public assistance status, veteran status, sexual orientation, gender identity, or gender expression. To learn more about diversity at the U: http://diversity.umn.edu

Any offer of employment is contingent upon the successful completion of a background check. Our presumption is that prospective employees are eligible to work here. Criminal convictions do not automatically disqualify finalists from employment.

The University of Minnesota, Twin Cities (UMTC)

The University of Minnesota, Twin Cities (UMTC), is among the largest public research universities in the country, offering undergraduate, graduate, and professional students a multitude of opportunities for study and research. Located at the heart of one of the nation's most vibrant, diverse metropolitan communities, students on the campuses in Minneapolis and St. Paul benefit from extensive partnerships with world-renowned health centers, international corporations, government agencies, and arts, nonprofit, and public service organizations.

At the University of Minnesota, we are proud to be recognized by the Star Tribune as a Top Workplace for 2021, as well as by Forbes as Best Employers for Women and one of America's Best Employers (2015, 2018, 2019, 2023), Best Employer for Diversity (2019, 2020), Best Employer for New Grads (2018, 2019), and Best Employer by State (2019, 2022).