Junior Specialist- Isseroff Lab

This position provides technical, scientific and organizational assistance to PI Dr. Roslyn Isseroff's research program in the Department of Dermatology, which focuses on tissue repair and regeneration, and wound healing. Dr. Isseroff is a clinician/physician-scientist who combines work as a dermatologist wound specialist with bench, translational and clinical research in wound healing, with a focus on generating novel therapies to improve healing using in vitro, in vivo animal models and clinical human trials. As such, the successful candidate will be exposed to generating novel technologies that can be translated to the care of patients with non-healing ulcers. The research is designed to better understand the biology of healing and how it can be accelerated. This project collaborates with faculty in immunology and animal science at UC Davis, and with engineering and computer scientists at UC Santa Cruz.

MAJOR RESPONSIBILITIES AND AREAS OF EXPERTISE

Under the direction of the PI, the candidate's responsibilities are a significant and essential part of publishable research activities. The candidate will assist the PI's funded studies of biophysical control of wound healing, with responsibilities that include preparation of samples for microbiome analysis, standard microbiological techniques, prokaryotic and eukaryotic cell culture, molecular biology techniques such as PCR and qPCR. Other responsibilities include preparation of tissues for histological and immunohistological examination, assisting with animal wound surgery. The candidate will work with the project team to collect data for manuscript preparation, review, and submission. The candidate's significant and meaningful contributions described above will lead to authorship or acknowledgement on published work. Candidates interested in human translation can be invited to join the PI's clinical research team and contribute to ongoing patient-centered studies.

90% RESEARCH

• Planning, performance and interpretation of PCR and qPCR on animal and human tissues.
• Embedding, sectioning and staining of animal and human tissues, and morphometric analysis of healing using computer assistance programs.
• Assisting as needed with animal surgeries, on small and large animal models.
• Create and manage a database of needed information, communicate with PI or team members to maintain and update inventory and order supplies, maintain computers, files, and programs with appropriate backup for all computers under supervision, development/revision of appropriate standard operating procedure (SOP) as needed.
• Maintaining meticulous documentation of experimental work.
• Contribute to scholarly manuscripts through reviewing and commenting on draft manuscripts, conducting literature searches, data collection, and helping to write methods sections
• Contribute original ideas for further investigation.
• Collect, organize, and summarize laboratory data. Evaluate data and prepare tables and graphs for publication. Calculate statistics as needed using SAS and/or Excel.
• Provide summary information for reports and sections of publications and presentations.
• Contribute to routine laboratory upkeep as directed by laboratory manager.
• Reading and interpreting the relevant literature, presenting summaries at lab meetings.

PROFESSIONAL COMPETENCE AND ACTIVITY (5% EFFORT)

• The candidate will have the potential to attend various scholarly conferences, such as the funding agency's quarterly reviews, and national meetings of the Wound Healing Society.
• The candidate will collaborate with researchers in the lab and will help to produce data presented at conferences.

UNIVERSITY AND PUBLIC SERVICE (5% EFFORT)

• Candidate may participate in activities of committees within the department, college, campus and other University entities, as appropriate.
• Candidate will be expected to mentor undergraduates in the lab, participate and contribute to laboratory and group meetings and other activities, as applicable

APPLICATION REQUIREMENTS

• Curriculum Vitae - Your most recently updated C.V.
• Cover Letter

Additional qualifications (required at time of start)

• Valid California driver's license.
• Must speak and write fluent English.
• Demonstrated experience with handling data in spreadsheets.
• Willingness and interest to learn new techniques.

Bachelor's Degree in biological sciences

• The job requires a highly motivated, organized, and focused individual who is eager to learn and to take initiative.
• This is a full-time appointment, with occasional evening or weekend hours expected for management of studies.
• Experience in mammalian DNA/ RNA extractions and PCR and quantitative real-time PCR.

• Experience in basic laboratory practices including sterile technique, serial dilutions, plating, pipetting, etc.
• Experience with aseptic and anaerobic technique
• Experience with maintaining laboratory supply and reagent inventory
• Familiarity with standard software (Microsoft Word, Excel, PowerPoint)
• Experience with operating liquid chromatographs (HPLC) and analyze HPLC data
• Excellent communication and organizational skills
• Knowledge of platingmethods for propagation, enumeration and isolation of bacterial species
• Species identification by the analysis of 16S rRNA gene sequences
• Experience with handling large animals (i.e. pigs)
• Comfortable working with biological human and animal samples.

• 3-4 required (contact information only)

As a condition of employment, the finalist will be required to disclose if they are subject to any final administrative or judicial decisions within the last seven years determining that they committed any misconduct, are currently being investigated for misconduct, left a position during an investigation for alleged misconduct, or have filed an appeal with a previous employer.

• "Misconduct" means any violation of the policies or laws governing conduct at the applicant's previous place of employment, including, but not limited to, violations of policies or laws prohibiting sexual harassment, sexual assault, or other forms of harassment, discrimination, dishonesty, or unethical conduct, as defined by the employer.
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• UC Anti-Discrimination Policy for Employees, Students and Third Parties
• APM - 035: Affirmative Action and Nondiscrimination in Employment

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